Effect of deer bone polypeptides on bone microarchitecture of dexamethasone-induced osteoporosis rats / 中草药

ABSTRACT

Objective: To investigate the effect of deer bone polypeptides on bone microarchitecture of dexamethasone-induced osteoporosis (GIOP) rats. Methods: The rats were im given dexamethasone for establishing a rat GIOP model. The rats were ig given deer bone polypeptide with different doses for 75 d. The serum biochemical indexes were classic detection. The three-dimensional analysis of the trabecular structure of proximal tibia was conducted by microCT method; The pathological changes in the morphology of distal femur were observed by hematoxylin. Results: Deer bone polypeptides could increase serum Ca2+ and bone-gla-protein (BGP), decrease serum P3+, alkaline phorphatase (ALP), and parathyroid (PTH) in rats with GIOP. Deer bone polypeptide showed a tendency to reduce the serum CT in rats with GIOP, but the decreasing tendency was not statistically significant compared with the blank control group. The trabecula was thin and ruptured, its free ends increased, its number decreased, its spaces were widened, and the destructed space structures increased in rats with GIOP, while the intervention with deer bone polypeptides could significantly improve the above indexes to evaluate the bone microstructure. Conclusion: Deer bone polypeptides can inhibit the glucocorticoids-induced metabolism imbalance of calcium and phosphorus, reduce the serum ALP, increase the serum BGP, and inhibit the bone resorption and bone formation in rats with GIOP. Moreover, deer bone polypeptides can improve pathological changes in the microstructure and protect osteoporosis rats.