Analysis of transicriptomes and differentially expressed genes in Eleutherococcus senticosus / 中草药

ABSTRACT

Objective: To obtain the transcriptome database and differentially expressed genes of Eleutherococcus senticosus. Methods: We choose the high content group and the low content group of saponin as experimental materials, and use the high-throughput sequencing technology (Illumina HiSeq 4000) to sequence the transcriptome of E. senticosus, then we systematically analyze the sequencing results in the bioinformatic way. Results: We have assembled 8.34 Gb database, after assembly steps, we get 77 087 of E. senticosus unigenes, then blasting them with five data banks. All unigenes are involved in 55 GO-terms and 116 metabolic pathways. Though the differentially expressed analysis of two materials, we get 530 differentially expressed genes, the up-regulated genes account for 42.08%, the down-regulated genes account for 57.92%. After GO and Pathway enrichment analysis, we get 408 GO-natations and 40 metabolic pathways. Conclusion: These data represent the abundant messeges about transcripts and provide the valuable genome data sources in molecular biology of E. senticosus.