Dynamic accumulations and distributions of eight chemical constituents of Hunan Belladonnae Herba in various parts and different growing periods / 中草药

ABSTRACT

Objective: To investigate the dynamic accumulations and distributions of eight chemical compounds of Belladonnae Herba in different growing periods and explore the distribution of each ingredient in various parts. Methods: The detection was performed by RP-HPLC with diode array detector (DAD). The mobile phase was acetonitrile-0.05% phosphonic acid in gradient elution. The HPLC method was established for the simultaneous determination of the eight compounds, such as scopolin, chlorogenic acid, quercetin-3-O-galactose (6→1) rhamnose-7-O-glucoside, quercetin-3-O-glucose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-galactose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-glucose-(6→1) rhamnose-7-O-glucoside, scopoletin, and rutin, and also for the the comparison on the changes of each ingredient in different growing periods and distribution in various parts. Results: The total amount of each ingredient in the whole plant increased along with its growth. However, each ingredient had different rate of increase, in addition, most compounds would reach to a balance of accumulation in the middle or the last third of June. The analysis results showed scopolin mainly distributed in the roots, while chlorogenic acid had a higher distribution in the leaves and flowers; Scopoletin distributed evenly among all parts in the plant, and the others possessed a higher distribution among the leaves and flowers, however, there was scare distribution in the roots. Conclusion: Each ingredient of Belladonnae Herba has different rate of increase and different distribution in each part, resulting in comparatively large difference of each compound. The experimental data of this research could provide the basis for the implantation, harvest and collection, and quality evaluation of Belladonnae Herba.