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Simultaneous determination of thirteen constituents in Jiuwei Qianghuo Oral Liquid by RP-HPLC / 中草药
Chinese Traditional and Herbal Drugs ; (24): 3824-3828, 2016.
Article in Chinese | WPRIM | ID: wpr-853185
ABSTRACT

Objective:

To establish an HPLC method for the simultaneous determination of catalpol, liquiritin, ammonium glycyrrhizinate, asarinin, verbascoside, atractylodin, ferulic acid, imperatorin, notopterol, isoimperatorin, baicalin, prim-O-glucosylcimifugin, and 5-O-methylvisammioside in Jiuwei Qianghuo Oral Liquid (JQOL).

Methods:

The analysis was performed on Zorbax Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) by gradient elution of acetonitrile-0.005 mol/L KH2PO4 (adjusting to pH 3.0 with phosphoric acid) (1585). The flow rate was 1.0 mL/min.

Results:

The linear ranges of catalpol, liquiritin, ammonium glycyrrhizinate, asarinin, verbascoside, atractylodin, ferulic acid, imperatorin, notopterol, isoimperatorin, baicalin, prim-O-glucosylcimifugin, and 5-O-methylvisammioside were 2.08-31.22 μg/mL (r=0.9995), 4.01-60.15 μg/mL (r=0.9992), 10.09-151.31 μg/mL (r=0.9992), 4.98-74.63 μg/mL (r=0.9994), 2.05-30.74 μg/mL (r=0.9996), 4.10-61.46 μg/mL (r=0.9996), 2.93-43.98 μg/mL (r=0.9993), 2.04-30.66 μg/mL (r=0.9995), 12.54-181.55 μg/mL (r=0.9997), 53.95-89.23 μg/mL (r=0.9995), 12.05-180.68 μg/mL (r=0.9995), 5.97-89.51 μg/mL (r=0.9994), and 7.99-119.82 μg/mL (r=0.9996). The average recoveries (n=6) were 98.8% (RSD=1.9%), 98.6% (RSD=1.8%), 101.2% (RSD=1.5%), 99.4% (RSD=0.8%), 100.1% (RSD=0.6%), 99.7% (RSD=0.9%), 98.9% (RSD=1.2%), 99.4% (RSD=2.0%), 100.5% (RSD=1.6%), 98.7% (RSD=0.8%), 101.2% (RSD=1.4%), 98.3% (RSD=1.5%), and 99.1% (RSD=1.7%), respectively. The contents of nine batches of the catalpol, liquiritin, ammonium glycyrrhizinate, asarinin, verbascoside, atractylodin, ferulic acid, imperatorin, notopterol, isoimperatorin, baicalin, prim-O-glucosylcimifugin, and 5-O-methylvisammioside were 0.229-0.259 mg/L, 1.231-1.260 mg/L, 0.849-0.877 mg/L, 0.357-0.371 mg/L, 0.149-0.169 mg/L, 0.941-0.967 mg/L, 0.529-0.547 mg/L, 0.269-0.294 mg/L, 1.039-1.067 mg/L, 0.043-0.064 mg/L, 3.631-3.649 mg/L, 0.157-0.183 mg/L, and 0.068-0.084 mg/L.

Conclusion:

This method is simple and rapid, and can be used for the quality control of JQOL with satisfactory separation and repeatability.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2016 Type: Article