Simultaneous determination of eight components in Jiawei Xiaoyao Pill by HPLC-DAD / 中草药

ABSTRACT

Objective: To establish an HPLC-DAD method for the simultaneous determination of eight components in Jiaowei Xiaoyao Pill (JXP), such as jasminoidin, liquiritin, paeonol, crocin I, crocin II, peoniflorin, glycyrrhizic acid, and ferulic acid. Methods: The chromatographic separation was performed on a Plastisil ODS column (250 mm×4.6 mm, 5 μm) with acetonitrile (B)-0.2% phosphate acid solution (D) as mobile phase at the flow rate of 1.0 mL/min for gradient elution and the column temperature was 37℃. The detection wavelength was set at 238 nm for jasminoidin, 274 nm for liquiritin and paeonol, 440 nm for crocin I and crocin II, 228 nm for peoniflorin and glycyrrhizic acid, and 324 nm for ferulic acid. The volume of sample injection was 10 μL. Results: The eight compounds were well separated under the determined chromatographic conditions. The RSD of precision and repeatability experiment were all less than 2% and the sample solution was stable during 12 h. All the compounds had a wide linear range and good linearity the linear range of jasminoidin, liquiritin, paeonol, crocin I, crocin II, peoniflorin, glycyrrhizic acid, and ferulic acid were 48-288 μg/mL (r=0.9994), 60-360 μg/mL (r=0.9992), 64-384 μg/mL (r=0.9998), 19.2-115.2 μg/mL (r=0.9995), 4-24 μg/mL (r=0.9992), 96-576 μg/mL (r=0.9996), 64-384 μg/mL (r=0.9997), and 20-120 μg/mL (r=0.9994), respectively. The average recoveries were between 99.13%-100.25% and the RSD were all less than 2.0%. The content ranges of jasminoidin, liquiritin, paeonol, crocin I, crocin II, peoniflorin, glycyrrhizic acid, and ferulic acid in six batches of JXP were 2.34-2.82 mg/g, 2.63-3.28 mg/g, 3.66-4.55 mg/g, 1.05-1.41 mg/g, 0.34-0.41 mg/g, 4.78-5.32 mg/g, 2.83-3.37 mg/g, and 1.36-1.73 mg/g, respectively. Conclusion: The method is simple and convenient, and the methodology validation shows that determination result of the method is accurate and reliable and it can be an effective approach for the quality control of JXP.