Effect of different drying methods on Ophiopogonis Radix quality based on chemical composition characteristics / 中草药

ABSTRACT

Objective: Using chemical component content determination and fingerprint analysis to evaluate the effects of different drying methods on quality of Ophiopogonis Radix. Methods: The contents of total saponin, total flavonoids, and total polysaccharide were determined by ultraviolet spectrophotometry, and ophiopogonin D and methylophiopogonanone A were determined using HPLC; the chromatograms fingerprint was established by HPLC. Results: The influence of different drying methods on the chemical components in Ophiopogonis Radixa was significantly different, and the coefficient of variation fell in the range of 6.9%-20.8%. Among them, the damage degree of dried in the shade, dried in the sun, freeze drying and dried after semi-died in the sun is small on chemical component of Ophiopogon japonicus. However, the far infrared drying and microwave drying have the most serious effect; Ophiopogonis Radix samples have 18 characteristic peaks through HPLC. The 14 kinds of drying methods were divided into three types by cluster analysis, and a class of drying methods has similar drying conditions. Conclusion: The difference of drying method can be effectively reflected by the change of the content of chemical compositions and the feature of HPLC spectrum, which can be used as indicators of O. japonicus origin screening method. In the producing area, it is best to combine drying in the sun with in air source heat pump dryer in processing of Ophiopogonis Radix.