Chemical constituents of ethyl acetate extract of Empetrum nigrum var. japonicum and their effect on alcoholic fatty liver of rats / 中草药

ABSTRACT

Objective: To study the chemical constituents from the ethyl acetate extract fraction of 70% ethanol extract in the aerial parts of northeastern crowberry (Empetrum nigrum var. japonicum) and their effect on the alcohol fatty liver (AFL) of rats, and to provide the scientific basis for the use and development of the northeastern crowberry. Methods: Chromatography separation technology and spectral analysis technology were used for the separation, purification, structure analysis, and identification. AFL model of SD rats was induced by 45% ethanol. To observe the effect of ethyl acetate extract in northeastern crowberry on the liver index, serology, and liver tissue homogenates in AFL of rats. Results: Eight compounds were isolated and identified as β-sitosterol (1), ursolic acid (2), uvaol (3), 2',4'-dihydroxy dihydrochalcone (4), 2',4'-dihydroxy chalcone (5), 7-hydroxy flavone (6), 4'-methoxy-2'-hydroxyl-dihydroxy chalcone (7), and isoliquiritigenin (8). Compared with the model group, giving the ethyl acetate extract of northeastern crowberry could decrease the liver index of rats, reduce serum lipid metabolism of TG, TC, reduce the serum enzyme ALT, AST, liver homogenate in oxidative stress, and increase the activities of SOD and GSH. Conclusion: Compounds 1-4, and 6-8 are isolated from E. nigrum var. japonicum for the first time. The effect of ethyl acetate extract in E. nigrum var. japonicum on AFL of rats is obvious.