Correlation study on HPLC characteristic spectrum of raw herb-decoction pieces-extract-dispensing granules of Angelicae Dahuricae Radix / 中草药

ABSTRACT

Objective: To establish an HPLC characteristic spectrum of raw herbs-decoction pieces-extract-dispensing granules of Angelicae Dahuricae Radix (ADR) and evaluate the correlation among them. Methods: Gradient elution was performed with mobile phase of acetonitrile-water, detection wavelength of 320 nm, flow rate of 1.0 mL/min, and column temperature of 25℃, by setting the characteristic spectrum as control and evaluating the correlation of raw herbs-decoction pieces-extract-dispensing granules. Results: There were 10 common characteristic peaks in all characteristic spectra of 10 batches of raw herbs, decoction pieces, extract, and dispensing granules of ADR, presenting a good correlation. Conclusion: This study established an HPLC characteristic spectrum and reflected the whole picture of multi-component of raw herbs, decoction pieces, extract, and dispensing granules of ADR provides the reference for quality control of dispensing granules of ADR.