Renal protection of Jiangzhi Tongluo Soft Capsule on membranous nephropathy rats and effects on expression of Bcl-2 and Bad in renal tissue / 中草药

ABSTRACT

Objective: To observe the effects of Jiangzhi Tongluo Soft Capsule on blood biochemical indexes and expression of Bcl-2 and Bad in membranous nephropathy (MN) rats, explore the renal protective effects and possible mechanism of Jiangzhi Tongluo Soft Capsule on MN rats. Methods: Total 60 SD rats were randomly selected, 10 for normal group, the other 50 for MN model establishment, then randomly divided into model group, Benazepril group, and Jiangzhi Tongluo Soft Capsule group (25, 50, and 100 mg/kg), each group was treated according to the corresponding dose. The 24 h urinary protein quantitative (UTP), serum total cholesterol (TC), triglyceride (TG), total protein (TP), albumin (ALB), blood urea nitrogen (BUN), and serum creatinine (Scr) in rats in the 4th weekend were tested; The renal pathological morphology changes were observed by electron microscope and immunofluorescence technique, and detecting the expression of Bcl-2, Bad by immunohistochemical and real-time PCR methods. Results: Compared with the model group, UTP, TC, and TG in the treatment groups were lower (P 0.05), and the effect was better than Jiangzhi Tongluo Soft Capsule 25 mg/kg group (P 0.05). Compared with the model group, the expression of Bcl-2 mRNA significantly raised (P 0.05), and the effect was better than that in Jiangzhi Tongluo Soft Capsule 25mg/kg group (P < 0.05). Conclusion: The renal protection of Jiangzhi Tongluo Soft Capsule on MN rats may be related to the function of raising the expression of Bcl-2, downgrading the expression of Bad, inhibiting the sertoli cell apoptosis, and repairing the damaged cells.