Optimization of extraction technology process for Honghua Tongluo Prescription by an FAHP-based evaluation model / 中草药

ABSTRACT

Objective: To establish an optimization model for the extraction technology process of Honghua Tongluo Prescription (HTP) based on fuzzy analytic hierarchy process (FAHP). Methods: The evaluation indexes tree was established which included objective level, criterion level, and index level. The criterion level consisted of effective constituent, impurity control, behavioristics, histopathology, and immune response. The index level consisted the extraction efficiency of hydroxysafflor yellow A (HSYA) and icariin, the content of total substances, mechano-allodynia, and mechano-hyperalgesia, the ratios of decentered nucleolus in dorsal root ganglion, the content of SP in pelma skin with plantar P quality as the index layer. Weighting coefficient of all the indexes was decided by FAHP based calculation. HTP extraction process route was comprehensively evaluated. Results: The importance degrees (ω) of each criterion from high to low were immune response (ω=0.245), histopathology (ω=0.23), effective constituent, behavioristics (ω=0.20), and impurity control (ω=0.125). The optimized extraction technology process was that Carthami Flos was better extracted by warm maceration with water at 60℃, Epimedii Herba was circulated by reflux extraction with water. Cinnamomi Ramulus was better treated by reflux extraction with 80% alcohol. Conclusion: An FAHP-based evaluation method with pharmacological indexes, effective constituent indexes, and impurity control as evaluation indexes, which could put formula Chinese materia medica principle and modern pharmaceutical engineering demands into consideration, is comprehensive and effective.