Chemical constituents from rhizomes of Rhodiola wallichiana var. cholaensis and their protective effects on myocardium / 中草药

ABSTRACT

Objective: To investigate the chemical constituents from the rhizomes of Rhodiola wallichiana var. cholaensis and their protective effects on myocardium of H9c2 cells. Methods: The compounds were isolated by dynamic axial compression chromatography, silica gel column chromatography, and HPLC. Their structures were identified on the basis of spectral data analysis. The protective effects of all isolated compounds on myocardium were determined. Results: Sixteen compounds were isolated from the rhizomes of R. wallichiana var. cholaensis and identified as salidroside (1), gallic acid (2), methyl gallate (3), quercetin (4), pyrogallol (5), 6″-O-galloylsalidroside (6), ethyl gallate (7), kaemnpferol-7-O-α-L-rhamnopyranoside (8), herbacertin-7-O-β-D-glucopyranoside (9), rhodiosin (10), kaemnpferol-3-O-β-D-glucopyranoside (11), herbacetin (12), herbacertin-7-α-L-rhamnopyranoside (13), tricin (14), rutin (15), and kaemnpferol-3-O-(2″-O-β-D-xylosyl)-β-D-glucopyranoside (16). Compounds 2, 6, 8, 12, and 15 showed the significant protective effects against H9c2 cells at the concentration of 25 μg/mL and the protective ratios were 20.40%, 31.54%, 67.61%, 44.27%, and 47.84%. Conclusion: Compound 5 is isolated from the species of genus Rhodiola L. for the first time, and compounds 7-16 are obtained from the plant for the first time. Compounds 2, 6, 8, 12, and 15 could protect the myocardium of H9c2 cells to some extent.