Methodology on specific knock out of glycyrrhizic acid by immunoaffinity chromatography column / 中草药

ABSTRACT

Objective: To prepare the glycyrrhizic acid (GA) immunoaffinity chromatography column, which could specifically knock out the GA. Methods: An immunoaffinity chromatography (IAC) column was developed by covalently coupling the anti-GA-MAb to CNBr-activated Sepharose™ 4B. The concentration of GA was detected by HPLC, and the maximum coupling capacity, stability, precision, and accuracy of the IAC column for GA were studied. Results: The maximum capacity of the IAC column for GA was 1.326 11 mg, the precision (RSD) of GA was 0.65%, and the accuracy (RE) was 0.37%. RSD of GA stayed 1.37% during 32 d, which showed a pretty well stability. Conclusion: GA-IAC column could rapidly, effectively, and stably knock out the GA.