"Correlation in quality and metabolism" of multicomponents in Angelicae Sinensis Radix / 中草药

ABSTRACT

Objective: To study the dynamic processes of in vivo metabolism of multicomponent in the decoction of Angelicae Sinensis Radix (ASR), select the quality control components, look for a new quality evaluation method for Chinese materia medica (CMM). Methods: The blood was taken from mesenteric vein and abdominal aorta. By analyzing HPLC fingerprints of intestinal metabolism and liver metabolism, we could infer multicomponent changes in the metabolic process of water extract from ASR. Results: Totally 15 components were basically stable in the digestive juice, four of them may be metabolized by intestine, while seven of them were metabolized by liver. And we found one new component was the metabolite of liver, seven new components were the metabolites of intestine, but five of them were metabolized by liver and could not be absorbed into blood. Finally, we identified that four prototype components of water extract from ASR and three metabolites could be absorbed into blood. Conclusion: Based on the multicomponent metabolic profiling of ASR, we initially select the four prototype components including senkyunolide I as the quality control component, and we finally establish a quality evaluation method for "correlation in quality and metabolism".