Screening of reference genes in Lonicera macranthoides and spatio-temporal expression analysis of LmAGL15 in Mads-box family / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2727-2733, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853377
ABSTRACT
Objective:
To screen the reference genes of Lonicera macranthoides for gene expression analysis and to study the spatio-temporal expression characteristics of LmAGL15 which was a member of Mads-Box family.Methods:
In this study, 18 S rRNA, Ubiquilin, Actin and Efl-β of L. macranthoides were cloned and the stabilities of the four housekeeping genes were evaluated in different positions (leaves, stems, and buds) and different periods of bud development. In addition, the spatio-temporal expression of LmAGL15 gene was analyzed.Results:
18 S rRNA was the most suitable reference gene for spatio-temporal expression analysis in L. macranthoides; The relative expression of LmAGL15 was low in leaves and stems, and that in buds was higher.Conclusion:
18 S rRNA is the most suitable reference gene in L. macranthoides. The relative expression of LmAGL15 changes significantly in leaves, stems, and buds.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS