Effect of Shenmai Iniection on isoforms of cytochrome P450 enzymes in rats / 中草药

ABSTRACT

Objective: To investigate the effects of Shenmai Injection (SMI) on activities of six isoforms of cytochrome P450 (CYP450) by Cocktail probe drugs in rats. Methods: SD rats were randomly divided into SM group and blank control group, which were given SMI (10 mL/kg) or normal saline for 7 d. Phenacetin, bupropion, tolbutamide, omeprazole, metoprolol, and midazolam were used as probe drugs for CYP1A2, CYP2B1, CYP2C9, CYP2C19, CYP2D6, and CYP3A4. The UPLC-MS/MS method was used to determine the concentration of probe drugs in rat plasma, and the pharmacokinetic parameters were estimated by DAS3.0. Results: Compared with the blank control group, AUC0~∞, CL, and Cmax of phenacetin, bupropion, and omeprazole were significantly decreased (P < 0.05). However, no significant difference of plasma concentration and pharmacokinetics for tolbutamide, metoprolol, and midazolam was shown between SMI group and the blank control group. Conclusion: SMI can inhibit CYPlA2, CYP2B1, and CYP2C19 activities significantly, but has no effect on the activities of CYP2C9, CYP2D6, and CYP3A4.