Subordinate function and principal component analysis of salt tolerance of seedlings germinated from microtubers of Jiangxi local yam / 中草药

ABSTRACT

Objective: The physiological regulation mechanism of seedlings germinated from Jiangxi local yam (Ruichang yam, Nancheng yam, Yongfeng yam, and Guangfeng yam) microtubers under salt stress was studied and the salt tolerance of seedlings germinated from microtubers of four kinds of local yams in Jiangxi was compared, which provided some references for salt tolerance mechanism research and salt tolerance breeding of four local yams in Jiangxi. Methods: Some physiological and biochemical index detection of seedlings germinated from Jiangxi local yam microtubers under salt stress were studied by spectrophotometer method. The subordinate function, principal component analysis (PCA), and clustering analysis of their salt tolerance were accomplished by fuzzy mathematics subordinate function formula and SPSS 19.0 software, respectively. Results: The total chlorophyll content and root activity of seedlings germinated from Jiangxi local yam microtubers under 0-300 mmol/L salt stress decreased significantly. The soluble protein content of seedlings germinated from Ruichang yam, Nancheng yam, and Guangfeng yam microtubers under 0-300 mmol/L salt stress increased firstly and then decreased, while the soluble protein content of seedlings germinated from Yongfeng yam microtubers under 0-300 mmol/L salt stress decreased significantly. The total soluble sugar content, proline content, MDA content and membrane permeability of seedlings germinated from Jiangxi local yam microtubers under 0-300 mmol/L salt stress significantly increased. The POD, SOD, and CAT activities of seedlings germinated from Jiangxi local yam microtubers under 0-300 mmol/L salt stress firstly increased and then decreased. Based on the subordinate function and PCA of SPSS 19.0 software, three principal components of 10 physiological and biochemical indexes of seedlings germinated from Jiangxi local yam microtubers under salt stress were induced. The salt tolerance order of seedlings germinated from Jiangxi local yam microtubers was Guangfeng yam > Yongfeng yam > Ruichang yam > Nancheng yam. Through the cluster analysis by SPSS 19.0 software, the salt resistance of four kinds of Jiangxi local yam is divided into two types, the salt tolerance of Guangfeng yam and Yongfeng yam was strong, and of Ruichang yam and Nancheng yam were sensitive to salt stress. Conclusion: The regulation mechanism of salt tolerance of seedlings germinated from four kinds of Jiangxi local yam microtubers is revealed and the objective evaluation of salt tolerance of four kinds of Jiangxi local yam is made in the paper, which will provide a theoretical basis for sowing in the field of four kinds of Jiangxi local yam microtubers.