Simultaneous determination of nine constituents in Asteris Radix by HPLC-MS/MS / 中草药

ABSTRACT

Objective: To develop an HPLC-MS/MS method for the simultaneous determination of nine constituents (kaempferol, quercetin, luteolin, isorhamnetin, scopoletin, umbelliferone, ferulic acid, caffeic acid, and chlorogenic acid) in Asteris Radix (the roots and rhizoma of Aster tataricus. Methods: Analysis was performed on a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) eluted with acetonitrile (0.05% formic acid) and water (0.05% formic acid) in a gradient program. The flow rate was 0.8 mL/min, the injection volume was 10 μL, and the column temperature was 30℃. The multiple-reaction monitoring scanning (MRM) was employed for the quantification with switching electrospray ion source polarity in negative mode. The ion spray voltage was set at -4 500 V and the turbo spray temperature was maintained at 650℃. Results: Kaempferol, quercetin, luteolin, isorhamnetin, scopoletin, umbelliferone, ferulic acid, caffeic acid, and chlorogenic acid showed a good linearity in the ranges of 0.970-30.30 (r = 0.998 7), 0.498-15.55 (r = 0.999 6), 0.014-0.438 (r = 0.999 5), 0.022-21.95 (r = 0.998 5), 0.011-1.100 (r = 0.999 5), 0.008-0.247 (r = 0.999 8), 0.194-6.050 (r = 0.999 6), 0.197-6.150 (r = 0.999 5), and 1.459-45.609 μg/mL (r = 0.999 0). The average recoveries varied from 97.43%-103.87%. The RSD values of precision varied from 1.95% - 3.09%. Conclusion: The method is simple, rapid, and sensitive. It could be used as a quantitative determination method for the nine components in A. tataricus.