HPLC fingerprint of Shufeng Jiedu Capsule / 中草药

ABSTRACT

Objective: To establish an HPLC method for the fingerprint analysis of Shufeng Jiedu Capsule (SJC), so as to provide evidence for the quality control of it. Methods: HPLC method was applied with the chromatographic condition as follows The chromatographic column was Unitary C18 column (200 mm×4.6 mm, 5 μm), acetonitrile-0.1% formic acid as the mobile phase with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 250 nm, the column temperature was 30℃, and the injection volume was 10 μL. The HPLC fingerprint of SJC was analyzed with similarity analysis and principal component analysis (PCA) method. Results: The fingerprint chromatography included 22 mutual peaks, of which eight mutual peaks from Polygonum cuspidatum, six mutual peaks from Forsythia suspensa, three mutual peaks from Verbena officinalis, two mutual peaks from Glycyrrhiza uralensis, and one mutual peak from Thlaspi arvense. The similarity among 14 batches was more than 0.9.Based on the HPLC-MS2 data, 16 components were identified, which were forsythosid E, hastatoside, verbenalin, 5'-hydroxy-forsythiaside A, polydatin, phillyrin, forsythosid A, acteoside, iso-forsythosid A, aloe-emodin, emodin-8-O-grapeglycosides, parietic acid, monoammonium glycyrrhizinate, 3-hydroxyglabrol, emodin, and chrysophanol. Conclusion: It is the first time to establish the HPLC fingerprint of SJC. The method is simple, accurate, and reproducible, which could reflect the chemical composition information of SJC comprehensively and provide scientific evidence for the quality control.