Study on expression of metallothionein 2 gene in safflower / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2152-2156, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853468
ABSTRACT
Objective:
To obtain the transgenic safflower plants which expressed Arabidopsis thaliana metallothionein 2 (MT2) gene, and lay a foundation for development of MT products.Methods:
The oleosin-MT gene was obtained from pEASY-oleosin-MT by Nco I/Hind III, then was inserted into plant expression vector pOP. The recombinant plasmid named pOP-oleosin-MT was transferred into Agrobacterium tumefaciens EHA105.The oleosin-MT gene was introduced into safflowers via Agrobacterium-mediated method and positive transgenic plants were determined by PCR analysis.Results:
The recombinant plasmid pOP-oleosin-MT was successfully constructed. PCR and Southern blotting analysis confirmed that MT gene was integrated into the genome of safflower plant and three transgenic plants were obtained.Conclusion:
The safflower regeneration system is constructed successfully and MT gene is successfully transformed into safflower plant.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
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