Effect of cold stress on expression characteristic of gene families of ginsenoside biosynthesis pathway / 中草药

ABSTRACT

Objective: To investigate the effect of cold stress on the three gene families (PgHMGR, PgSSs, and PgSEs) of the ginsenoside biosynthetic pathway, and to explore the mechanism of genes response to cold stress and find the key genes. Methods: The cold stress treatment was performed by selecting 3 weeks fresh callus which placed in the 5℃ refrigerator, and harvested after 0, 0.5, 1, 2, and 3 d treatment for further analysis, were recorded as CK, D1, D2, D3, and D4, respectively. Results: The expression of PgHMGR1 reached 1.3 times compared to the control group at D3 period, and the expression of PgHMGR2 reached the peak at D1 period, which was 3.8 times of the control group; the expression of PgSS1 was 1.7 times of the control group (D3 period); and the expressions of PgSE2 and PgSE1 were 6.9 and 6 times higher than those of the control group, respectively. There was no significant change of the expression of PgSS2, PgSE3, and PgHMGR3.Conclusion: The gene families of ginsenoside biosynthesis pathway positively response to cold stress treatment, and PgHMGR1, PgHMGR2, PgSS1, PgSE1, and PgSE2 may be the key family genes when Panax ginseng callus response to cold stress.