Studies on chemical constituents from Artabotrys pilosus / 中草药

ABSTRACT

Objective: To study the chemical constituents from twigs and leaves of Artabotrys pilosus. Methods: The chemical constituents of A. pilosus were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Sixteen compounds were isolated from the acetic ether fraction of 90% ethanol extract from the twigs and leaves of A. pilosus, and identified as dammaradienylacetate (1), lupeol (2), lupenyl acetate (3), betulinic acid (4), uvaol (5), ursolic acid (6), β-amyrin (7), erythrodiol (8), friedelinol (9), friedelin (10), α-spinasterone (11), stigmast-7-ene-3β-ol (12), stigmast-5-ene-3β-ol-7-one (13), stigmastan-3,6-diketone (14), stigmasterol (15), and β-sitosterol (16). Conclusion: All compounds are isolated from A. pilosus for the first time. Except for compounds 15 and 16, other compounds are isolated from the plants of Artabotrys R. Br. for the first time.