GC fingerprint of volatile components in Qishen Yiqi Dropping Pills / 中草药

ABSTRACT

Objective:To establish the GC fingerprint of Qishen Yiqi Dropping Pills(QYDP)and Dalbergiaodorifera oiland identify the common chromatographic peaks by GC-MS. Methods: The GC analysis was performed on an Agilent HP-INNOWaxcapillary gas chromatography column (30 m × 0.25 mm, 0.25 μm). The temperature program was as follows 100 ℃ for 2 min, 160 ℃ at 3 ℃/min, 190 ℃ at 1 ℃/min, 220 ℃ at 5 ℃/min, then 250 ℃ at 10 ℃/min; Nitrogen was used as the carrier gas with its flow rate 0.5 mL/min; The sample injection temperature was 250 ℃ and the split ratio was 51; The detector was FID with its temperature of 300 ℃. MS parameters MS electron energy of 70 eV, ion source temperature of 230 ℃, full-ion scan mass rangem/z 35-600, solvent delay for 8 min. Results: Thirty-three common peaks were found from QYDP and 25 common peaks were found from D.odorifera oil in their GC fingerprints. Twenty-three common peaks in the fingerprint ofD.odorifera oil,such as trans-nerolidol,3,7,11-trimethyl-3,6-epoxy-1,10-dodecadien-7-ol isomers, farnesol isomers, α-santalene, norbornane, andβ-bisabolene were identified. The similarity of all batches of QYDP and D.odorifera oil were both over 0.979. Conclusion: This method is simple and reliable and can be used to evaluate the quality of QYDP and D.odorifera oil.