Comparative study on HPLC characteristic spectra of Humuli Scandentis Herba from different habitats in Hebei province / 中草药

ABSTRACT

Objective: To establish and compare the HPLC characteristic spectra of Humuli Scandentis Herba form different habitats in Hebei province. Methods: HPLC was performed on Agilent 5 TC-C18 (250 mm × 4.6 mm, 5 μm) column, with the mobile phase of acetonitrile -0.2% phosphoric acid at flow rate of 1.0 mL/min; Detection wavelength was 340 nm; The column temperature was 25 ℃ and the sample size was 10 μL. Eleven batches of Humuli Scandentis Herba samples form different habitats in Hebei province were determined and the characteristic spectra of those were established. The fingerprint evaluation software (2004 edition) for Chinese materia medica (CMM) was used to evaluate the similarity of the 11 batches of samples. Results: There were nine characteristic peaks identified in the characteristic spectra of Humuli Scandentis Herba samples. Peak 7 was luteoloside and Peak 8 was apigenin-7-O-β-D-glucosidase. The similarities of seven batches of Humuli Scandentis Herba samples were proved to be higher than 0.900 and four batches of them were proved to be less than 0.900. Conclusion: The method is simple, accurate, and reproducible, and can provide the scientific evidence for controlling the internal quality standards effectively.