Simultaneous determination of chlorogenic acid, rutin, quercetin, and luteolin in kunlun snow daisy for uighur medicine by RP-HPLC / 中草药

ABSTRACT

Objective: The high performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of chlorogenic acid, rutin, quercetin, and luteolin in Kunlun snow daisy (Coreopsis tinctoria, Coreopsis Tinctoriae Folium). Methods: Agilent HC-C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was CH3OH (A)-0.01% H3PO4 (B) for the gradient elution with flow rate of 1.0 mL/min, the detection wavelength was 315 nm and the column temperature was 30 ℃. Results: There was a linear correlation between the concentration of chlorogenic acid 2.0-60 μg/mL (r = 0.999 8), rutin 5.0-100 μg/mL (r = 0.999 9), quercetin 1.0-40 μg/mL (r = 0.999 9), and luteolin 0.5-40 μg/mL (r = 0.999 9). The average recovery rates of chlorogenic acid, rutin, quercetin, and luteolin were 102.4% (RSD = 1.05%, n = 5), 98.6% (RSD = 1.28%, n = 5), 103.6% (RSD = 0.95%, n = 5), and 101.2% (RSD = 1.12%, n = 5), respectively. Conclusion: The analysis method is simple, rapid, reproducible, accurate, and reliable. It could be used to identify and evaluate the quantitative determination of chlorogenic acid, rutin, quercetin, and luteolin contents in Kunlun snow daisy.