Comparative study on effect of different extracts from carbonized Rubiae Radix et Rhizoma in acute blood stasis model of rats / 中草药

ABSTRACT

Objective: To observe the effect of different extracts from carbonized Rubiae Radix et Rhizoma (RRR) on acute blood stasis model of rats, in order to screen the optimal extraction method for its hemostatic effect by removing blood stasis. Methods: The acute blood stasis models of rats were established with sc injection of high-dose adrenaline hydrochloride after continuous administration for 8 d and being socked in ice-water, using Yunnan Baiyao as positive drug. The effect of the alcohol extract, compound (alcohol + aqueous) extract, aqueous extract, and aqueous extract after alcohol extracting on hemorheology, in vitro thrombus, blood plotelets system, and fibrinolytic system in acute blood stasis model of rats were observed. Results: Compared with the control group, the whole blood viscosity in each shear rate (P < 0.01) and the wet and dry weight of in vitro thrombus could be increased (P < 0.05), the coagulation time was prolonged (P < 0.05, 0.01), the FIB content and platelet aggregation rate induced by ADP increased (P < 0.01), the PAI-1 and TXB2 contents increased, and the 6-keto-PGF1α content decreased in the rats of model group (P < 0.05, 0.01). Compared with the model group, both the alcohol extract and compound extract could significantly improve the whole blood viscosity, decrease the wet and dry weight of external thrombus, shorten the coagulation time, increase the contents of PAI-1 and 6-keto-PGF1α, and decrease the TXB2 content (P < 0.05, 0.01). Both of them could make platelet aggregation rate increased, but have no significant influence on the content of FIB. The alcohol extract significantly decreased the content of t-PA (P < 0.05), while the compound extract significantly increased t-PA content (P < 0.01). The compound extract has influenced the hemorheology and the wet and dry weight of in vitro thrombus greater than the alcohol extract. While on the coagulation time, the effect of alcohol extract is greater. Conclusion: The hemostatic effects by removing blood stasis of alcohol extract and compound extract are stronger than those of others, especially the compound extract. In other words, the optimal extraction method of its hemostatic effect by removing blood stasis is the compound extract.