Determination of ergosterol in Lasiosphaera Seu Calvatia in various species from different habitats by HPLC / 中草药

ABSTRACT

Objective: The HPLC method was established for assessment of quality of Lasiosphaera seu Calvatia (LSC) based on the determined indicator of ergosterol. The contents of ergosterol in 42 batches of LSC were comparatively analyzed. Methods: The contents of ergosterol in LSC from different origins were determined by HPLC. The conditions were as follows BDS Hypersil C18 column (250 mm × 4.6 mm, 5 μm); flow rate 1.0 mL/min; detection wavelength 282 nm; column temperature 30 ℃; injection volume 20 μL; mobile phase acetonitrile (A) 0.1% formic acid (B) as the mobile phase gradient elution 0-15 min, 95% A, 15-50 min, 100% A. Results: The HPLC method to determine the ergosterol content of puffball was established.The ranges of ergosterol contents among different samples was 0.009 9%-0.150 3%. Conclusion: The contents of ergosterol in different LSC are obviously different, and the quality of commercial LSC is not coincident. So the quality control of LSC a needs further normalization and standardization. The data in this paper would provide a reasonable reference for the quality control of LSC.