ISSR analysis on genetic diversity of Paeonia suffruticosa in Hunan province / 中草药

ABSTRACT

Objective: In order to understand the genetic relationship and genetic diversity of peony (Paeonia suffruticosa) in Hunan province, the DNA fingerprints of 47 peony germplasm materials of Hunan province were studied. Methods: Seven primers selected from 100 ISSR primers could obtain high polymorphism and reproducibility bands using ISSR markers. Results: Total 77 DNA bands were amplified including 67 polymarphic bands, counting for 87.01%. Genetic similarity analysis showed that Hunan peony germplasm resourses with high genetic diversity. Through the computer software analysis, peony variety tested the average effective number of alleles was 1.395 4, the average Nei's genetic diversity index is 0.248 0, the average Shannon's information index was 0.385 9; Between each type of the similarity coefficient of Jaccard was 0.532 5-0.961 0, with an average of 0.751 5, which was a sign of close genetic relationship between the materials. These 47 germplasm resourses were divided into two groups and three subgroups by UPGMA analysis, which could distinguish the varieties from different sources. Conclusion: It reveals from the molecular level of Hunan local peony varieties, genetic relationship, and genetic diversity so as to provides the theoretical basis for the varieties breeding worke of heat and humid resistance of peony.