Research on compatibility mechanism of Mongolian medicine Sendeng-4 based on metabonomic methods / 中草药

ABSTRACT

Objective: To explore the mechanism of the compatibility of Mongolian medicine Sendeng-4. Methods: Sendeng-4 was composed of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction with Gardeniae Fructus, Toosendan Fructus, and Chebulae Fructus with a mass ratio of 5311. BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used as the chromatographic column, water (0.1% formic acid)-methanol were used as mobile phase for the gradient elution, and the ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS), principle component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the chemical constituent changes of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction. Results: The significant differences between the group of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and Sendeng-4 were observed in PCA and OPLS-DA model. There were significant differences among eight chemical compounds after compatibility (P<0.05). The relative contents of catechin, (-)-gallocatechin, dihydromyricetin, quercetin decreased, xanthocerasic acid, 3β-hydroxytirucalla-7,24-dien-21-oic acid, 3-oxotirucalla-7,24-dien-21-oic acid, and rutin were increased. Conclusion: Maybe the anti-inflammatory and anti-rheumatic activity of Sendeng-4 have the relationship with the content changes of some index component in Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium after compatibility.