UPLC fingerprint of Viciae Amoenae Herba / 中草药

ABSTRACT

Objective: To establish the ultra performance liquid chromatography (UPLC) fingerprint of Vicia amoena var. angusta and compare the other five different origin varieties of Viciae Amoenae Herba in order to evaluate the quality of the preparation. Methods: Eclipse Plus C18 RRHD column (50 mm×2.1 mm, 1.8 μm) were used with acetonitrile (B) and 0.1% formic acid aqueous solution (A) in gradient elution mode at a flow rate of 0.2 mL/min. The detection wavelength was set at 340 nm and the column was 30℃. The fingerprints for V. amoena var. angusta from 10 different areas were set up, the similarity assay for V. amoena var. angusta from ten different areas was carried out to evaluate their quality by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2004A edition), and the peaks were identified by comparing the reference substance with LC-MS/MS; Whilie the other origins of five kinds of varieties of Viciae Amoenae Herba were compared and identified in the same condition. Results: UPLC fingerprints for V. amoena var. angusta were established, six chromatographic peaks among 15 common peaks were identified by UPLC-ESI-Q-TOF-MS and be intended to be chlorogenic acid, myricitrin, hyperoside, isoquercitrin, kaempferit-rin, and quercitrin. UPLC fingerprint mutual pattern of other five kinds of origins varieties of Viciae Amoenae Herba and V. amoena var. angusta had obvious differences. Conclusion: This method is simple, rapid, stable, and reproducb1e, and could be used for the full quality evaluation of Viciae Amoenae Herba.