Analysis on chemical constituents in Hedera nepalensis by UPLC/Q-TOF-MS/MS / 中草药

ABSTRACT

Objective: Using UPLC/Q-TOF-MS/MS to analyze and identify the chemical constituents from the stems and leaves of Hedera nepalensis. Methods: The analysis was performed on an Welch C18 reverse phase column (100 mm×2.1 mm, 1.7 μm). The mobile phase consisted of acetonitrile (B) and 0.1% formic acid (A) was used as gradient elute. The flow rate was 0.3 mL/min gradient elution and column temperature was 40℃, the injection volume was 5 uL, MS conditions were ESI, positive and negative ion mode scanning. Results: Under the optimized condition, based on the database of Scifinder, MS/MS of standards and compared with reference results, 43 compounds were identified, including triterpenoid saponins, flavonoid glycosides, phenylpropanoids, and nucleotides. Conclusion: By using UPLC/Q-TOF-MS/MS method the main chemical constituents from H. nepalensis can be rapidly and accurately identified.