Research on separation and purification of extract from Dracocephalum moldevica with macroporous resins / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 599-605, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853701
ABSTRACT
Objective:
To investigate the technology for the separation and purification of extract in Dracocephalum moldevica (EDM) by macroporous resin.Methods:
Static and dynamic adsorption-desorption were used to select the best one from seven different type macroporous resins; With the content of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid as indexes, the purification technology parameters of EDM were optimized.Results:
HPD600 resin showed the best purifying profile, its optimum technology conditions were as follows The optimum concentration of the sample liquid was 0.08 g/mL equivalent to raw material, the resin column diameter-height ratio was 19, the amount of used adsorption was 0.32 g dried medicinal herb/mL resin, sample flow rate was 1.5 BV/h, and adsorption time was 12 h. In the course of elution, the resin column chromatography was eluted with 6 BV of 70% ethanol after removing impurities with 4 BV of water by flow rate of 1.5 BV/h. The contents of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid were more than 53%, 5.5%, 4.7%, and 2.5%.Conclusion:
Macroporous resin HPD600 is suitable to separate and purify EDM.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
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