Inhibition of butanol extract from Paris polyphylla var. yunnanensis against Candida albicans biofilm formation / 中草药

ABSTRACT

Objective: To investigate the effects of butanol extract from Paris polyphylla var. yunnanensis (BEPP) on the biofilm formation of Candida albicans in vitro. Methods: Microdilution method was used to determine the minimum inhibitory concentration (MIC). XTT assay was used to determine the SMIC80. Flat band method was used to protract the time-kill curve. Scanning electron microscopy (SEM) was used to observe the morphological changes of the biofilm. Confocal laser scanning microscopy (CLSM) was used to detect the thickness of the biofilm. The quantification real-time PCR (qRT-PCR) was used to detect the expression changes of genes (EAP1, MP65, and SUN41) of the biofilm cells. Results: The MIC of BEPP against C. albicans strains was determined as 32-128 μg/mL. The SMIC80 of BEPP against the biofilm of C. albicans strains was determined as 128-512 μg/mL. Time-sterilization curve results indicated that BEPP had a promise bactericidal effect. SEM results showed that the formation of C. albicans biofilm was inhibited by BEPP, and the morphology of biofilm was also affected by BEPP. The thickness of C. albicans biofilm was reduced by BEPP according to CLSM results. Furthermore, qRT-PCR results indicated that expressions of EAP1, MP65, and SUN41 were significantly down-regulated by BEPP. Conclusion: Our results demonstrate that BEPP inhibits the biofilm formation of C. albicans in vitro.