Protection of Compound Granules of Yinxingye on H9c2 myocardial cells injury induced by H2O2 / 中草药

ABSTRACT

Objective: To study the effects of Compound Granules of Yinxingye (Ginkgo leaf) on H2O2-induced cellular injury in H9c2 myocardial cells of rats. Methods: H9c2 cells were treated with H2O2 to establish a myocardial injury model, and then pretreated by Compound Granules of Yinxingye at the concentration of 25, 50, 100, and 200 μg/mL for 24 h. Cell viability was measured by methyl thiazolyl tetrazolium (MTT). Morphological changes were observed by phase contrast microscope. Lactic dehydrogenase (LDH), creatine kinase-MB (CK-MB), malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) levels were detected by biochemical kits. Intracellular reactive oxygen species and calcium ion concentration and mitochondrial membrane potential were measured by laser confocal microscopy. Results: The exposure of H9c2 cells to 500 μmol/L H2O2 for 6 h could reduce cell viability significantly and induce oxidative stress injury. The Compound Granules of Yinxingye pretreatment could improve the cell viability, improve the damage cell morphology change, reduce LDH, CK, MDA, and NO levels in the cell supernatant, increase SOD activity. ROS generation and concentration of intracellular calcium could be reduced and the in mitochondrial membrane potential declined. Conclusion: The Compound Granules of Yinxingye has the protective effect on H2O2-induced cellular injury in H9c2 myocardial cells.