Genetic diversity of wild plants in Rhodiola L. with two molecular marker methods of RAPD and ISSR / 中草药

ABSTRACT

Objective: The interspecific genetic relationships of the plants in Rhodiola L. were analyzed by RAPD and ISSR molecular markers. Methods: The genomic DNA was extracted by CTAB method. Eleven RAPD primers and 11 ISSR primers were selected to analyze the genetic diversities of four kinds of wild plants in Rhodiola L., which were obtained from different regions of China. Results: A total of 96 bands were amplified by 11 RAPD primers, and the percentage of polymorphism was 90.62%; And 102 bands were amplified by 11 ISSR primers, and the percentage of polymorphism was 100%. So the polymorphism detection ability of ISSR marker is higher than that of RAPD marker. Clustering analysis indicated that the samples were clustered into three categories by ISSR, RAPD + ISSR, and four categories by RAPD. Conclusion: Both ISSR and RAPD markers are efficient methods at revealing in interspecific or intraspecific genetic differences and diversity of the plants in Rhodiola L.