Protective effects and mechanism of extract from Cajanus cajan leaves against H2O2-induced oxidative injury on H9c2 cardiomyoblasts / 中草药

ABSTRACT

Objective: To investigate the protective effects and mechanism of the extract from Cajanus cajan leaves (ECCL) against H2O2-induced oxidative injury in H9c2 cardiomyoblasts. Methods: A model of H2O2-induced injury in H9c2 cardiomyoblasts was established. Cell viability was determined colorimetrically by MTT assay. The supernates and cells were collected, respectively, after the different treatments for measuring the LDH, MDA, and SOD levels with the corresponding detection kit according to the manufacturer's instructions. Western blotting was performed to exam the expression of p-Akt and p-eNOS in H9c2 cells respectively. Results: Compared with H2O2 group, the cell viability was increased significantly in ECCL + H2O2 groups (P < 0.01). The activity of LDH in the culture medium was decreased significantly (P < 0.05). The content of MDA in the culture medium was decreased significantly (P < 0.05). The activity of SOD was increased significantly (P < 0.01). Treated with ECCL, the expressions of p-Akt and p-eNOS in H9c2 cells injured from H2O2 were increased significantly (P < 0.01), When LY294002 (inhibitor of PI3K) was added, the effects of ECCL were cancelled. Conclusion: ECCL protects H9c2 cells against H2O2-induced oxidative injury partly through PI3K signaling pathway.