Chemical constituents from stems of Clausena lansium / 中草药

ABSTRACT

Objective: To study the chemical constituents from the stems of Clausena lansium. Methods: The chemical constituents were separated and purified by macroporous resin, silica gel, ODS column chromatography, Sephadex LH-20, and preparative HPLC. Their structures were determined by the analyses of ultraviolet spectrum, mass spectrum, and nuclear magnetic resonance spectroscopy. Results: Fifteen compounds were isolated from the n-BuOH fractions of 95% ethanol extract from the stems of C. lansium, and their structures were identified as 1, 1', 1″, 1‴, 1″″-tricontane lactam (1), 4-hydroxy-2, 6-dimethoxyphenyl 6'-O-syringoyl-β-D- glucopyranoside (2), 4-hydroxy-2, 6-dimethoxyphenyl 6'-O-vanilloyl-β-D-glucopyranoside (3), 4-hydroxymethyl-2-methoxyphenyl- 6'-O-syringoyl-β-D-glucopyranoside (4), 4-hydroxy-2-methoxyphenyl-6-O-syringoyl-β-D-glucopyranoside (5), syingin (6), coniferin (7), 3, 4, 5-trimethoxyphenyl-O-β-D-glucopyranoside (8), tinotuberide (9), trans-isoconiferin (10), phenethyl-O-β-D-glucopuranoside (11), araliopsine (12), geibalansine (13), integriquinolone (14), and γ-fagarine (15). Conclusion: Compounds 1-14 are isolated from this plant for the first time, compounds 1-11 are isolated from the plants of genus Clausena L. for the first time, and compound 1 is a new natural product.