Chemical constituents from Mangifera indica seed kernel and their effects on sperm quality of asthenospermia rats in vitro / 中草药

ABSTRACT

Objective: To study the chemical constituents of mango (Mangifera indica) seed kernel and their effects on sperm quality of asthenospermia rats in vitro. Methods: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, and recrystallization. Their structures were elucidated by spectral analyses such as 1H-NMR, 13C-NMR, and MS. The rats were treated with a single ig administration tripterygium glycosides to induce asthenospermia rat model, so that the effects of the compounds could be evaluated on the sperm motility and sperm survival rate of asthenospermia rats in vitro. Results: Nine compounds were isolated and purified from mango seed kernel, whose structures were elucidated as vitamin E (1), methyl gallate (2), kaempferol (3), quercetin (4), 3,4-O-isopropylidene shikimic acid (5), 5-hydroxy-4-oxopyran-2-carboxylic acid (6), ferulic acid (7), isoquercitrin (8), and 1,2,3,4,6-penta-O-galloylglucose (9). Compounds 1, 7, and 9 could increase the sperm motility and sperm survival rate of asthenospermia rats in vitro. Conclusion: Compounds 1, 3-6 and 8 are obtained from mango seed kernel for the first time; Compounds 1, 7, and 9 could improve the sperm quality of asthenospermia rats in vitro.