Simultaneous determination of 17 major components of Shuang-Huang-Lian Oral Liquid by UPLC-MS/MS / 中草药

ABSTRACT

Objective: To establish a UPLC-MS/MS method for simultaneous determination of 17 bioactive compounds in Shuang-Huang-Lian Oral Liquid. Methods: The separation was performed on an Acquity UPLC HSS T3 C18 column (100 mm×2.1 mm, 1.7 μm) and the flow rate was set at 0.4 mL/min with acetonitrile/methanol (41)-0.4% formic acid as the mobile phase. The column temperature was 40℃ and only 7 min was needed for separating the five pairs of isomers. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive and negative modes. Results: All calibration curves had good linearity (r2>0.9901). The precisions and accuracies of the 17 analytes were all satisfied and the recoveries were 95.81%-102.42%. The content ranges of the 17 compounds were 782.68-1034.27μg/mL for neochlorogenic acid, 786.35-1103.77 μg/mL for chlorogenic acid, 898.00-1238.94 μg/mL for cryptochlorogenic acid, 82.85-115.17 μg/mL for caffeic acid, 226.02-461.63 μg/mL for 3,5-dicaffeoylquinic acid, 191.59-404.21 μg/mL for 3,4-dicaffeoylquinic acid, 243.62-298.51 μg/mL for isoforsythiaside, 978.43-1487.37 μg/mL for forsythoside A, 351.97-435.82 μg/mL for forsythin, 41.19-75.65 μg/mL for rutin, 40.28-73.73 μg/mL for baicalein, 10080.54-10820.35 μg/mL for baicalin, 40.88-50.51 μg/mL for scutellarin, 187.73-204.85 μg/mL for wogonoside, 144.92-335.08 μg/mL for oroxylin A-7-O-β-D-glucuronide, 9.30-25.58 μg/mL for wogonin, and 7.51-16.58 μg/mL for oroxylin A, respectively. Conclusion: The developed UPLC-MS/MS method is simple, sensitive, and accurate, and could be applied for simultaneous determination of the 17 components in Shuang-Huang-Lian Oral Liquid.