Polymorphism analysis of endangered notopterygium incisum and endemic species based on ccpDNA trnT-trnL sequences / 中草药

ABSTRACT

Objective: To analyze the genetic diversity and genetic structure of 245 plant samples in Notopterygium incisum from different regions. Methods: Genetic diversity and genetic structure of 245 samples of N. incisum were studied using chloroplast cpDNA trnT-trnL direct sequencing method and Neighbor-joining (NJ) method. Results: Haplotype diversity (Hd) and nucleotide diversity (Pi) at the species level were 0.873 and 0.00407, respectively. At the population level, Hd ranged with 0.000-0.900, and Pi ranged with 0.000-0.0544. It indicated that N. incisum had a moderate level of genetic diversity than N. franchetii plants of the genus Notopterygium Boiss. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (77.55%) existed among populations, and gene flow was low (Nm=0.145) among populations. Based on the NJ tree, the 31 wild populations tested were clustered into two groups. Conclusion: N. incisum has the midium cpDNA diversity and higher population genetic differentiation.