Screening of strains with high content of secondary production in endophytic fungi and fermentation liquid from wild soybean / 中草药

ABSTRACT

Objective: Using 42 endophytes separated and purified from wild soybean as materials, the accumulation of secondary metabolites in hypha and fermentation liquid of endophytic fungi was analyzed so as to screen the strains with high content of secondary production. Methods: Liquid fermentation cultivation with shaking flask and ultraviolet spectrophotometer was utilized to detect the contents of total triterpenoids, flavonoids, polysaccharides, polyphenols, and total phenolic in different endophyte mycelia and their ferment liquids. Results: For 42 endophytic fungi from wild soybean, strains Y2S9, WDL3, and WDL2 had high content of polysaccharide up to 85.51 mg/g, then Y1S9 and Y6S8 had higher content of the flavonoid with 2.99 and 2.24 mg/g, respectively; In addition Y6S8, Y2R1, and Y6R2 would produce more triterpenoid with 25.55, 17.89, and 16.39 mg/g; Meanwhile WDS7 had the highest content of total phenol with 22.65 mg/g. For the fermentation liquid of endophytic fungi, strains Y1S8 and SYS4 had higher contents of flavonoid with 3.111 and 4.809 mg/L; But strains YSYL2, Y1S8, and WDL3 had the higher levels of total triterpenoids up to 17.46, 35.00, and 42.98 mg/L, respectively. Conclusion: Different habitats might be the main factor causing difference in the contents of secondary production. Eleven strains with high yield secondary metabolites selected from wild soybean would lay the foundation for mining endophytic fungi function and screening new drugs.