Distribution of salvianolic acids in aerial parts of Salvia miltiorrhiza during different growing periods and accumulation dynamic analysis / 中草药

ABSTRACT

Objective: To study the dynamic changes of aerial parts of Salvia miltiorrhiza during different growth periods based on the contents of seven kinds of salvianolic acids, and determine the value of aerial parts of S. miltiorrhiza. Methods: UPLC-TQ/MS method was applied. The separation was performed on Acquity UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm). The mobile phase was composed of acetonitrile and 0.1% aqueous formic acid by gradient elution. The flow rate of the mobile phase was 0.4 mL/min, and the column temperature was at 35℃. Results: The stems and leaves of S. miltiorrhiza were rich in salvianolic acids, but tanshinones were not detected in them. The content of salvianolic acids in stems and leaves were the highest in July or August besides salvianolic acid A and gradually dropped off till the lowest in December. The content of salvianolic acids in flowers reached maximum during the full-bloom stage. The dynamic change trend is not obvious before full-bloom stage, and its content sharply dropped when flowers began to fade. Conclusion: The aerial parts of S. miltiorrhiza were abundant in salvianolic acids, especially rosmarinic acid and salvianolic acid B, and the contents in luxuriant growth period were significantly higher than those in radix and rhizome of S. miltiorrhiza, which showed that the aerial parts were the important resources to obtain the salvianolic acids.