Distribution of the Bovine Lens Antigen in the Ocular Tissues

ABSTRACT

Distribution of the antigens of soluble lens protein in the bovine ocular tissues was studied by Ouchterlony's double agar diffusion technique. Guinea pig anti-bovine-lens-serum was prepared by 4 weekly injections of the soluble protein of the bovine lens with complete Freund adjuvant. Three precipitation lines were observed between lens antigen and antiserum after 36 hours diffusion, and two precipitation lines on iris antigen and two precipitation lines on antigen mixture of the pigment epithelium and the choroid were observed after 36 hours diffusion. No precipitation line was observed on cornea antigen, vitreous antigen and retina antigen.