Optimization of technology of freeze-dried powder of various fractions from Pheretima by Box-Behnken design and response surface method / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3166-3172, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-853886
ABSTRACT
Objective:
To optimize the technology of freeze-dried powder of various fractions from lumbricus (Pheretima) by Box-Behnken design and response surface method.Methods:
Using single factor experiment with Box-Behnken response surface method, freeze-dried rate as the index, the effects of sublimation temperature, sublimation time, analytical drying temperature, and drying time on the freeze-drying process were observed; The freeze-drying process of the active components in lumbricus was optimized; Using HPLC method, the fingerprint of the sample by the optimized technology was investigated, and the similarity of fingerprint was compared with the earthworm medicine, and the optimum freeze-drying process was obtained.Results:
The best freeze-drying process conditions of active components in lumbricus were as follows frozen temperature of -26.5 ℃, precool for 4 h, sublimation drying temperature of -20 ℃, sublimation time of 7 h, analytical drying temperature of 30 ℃, analytical drying time of 3.5 h, freeze-dried rate of 96.55%, and compared with medicinal materials of amino acid, the similarities of fingerprint were both greater than 0.9.Conclusion:
Box-Behnken response surface method is used for the freeze-drying process conditions of active components in the lumbricus, the optimization is feasible, the effect of model is good, and this optimization process has the feasibility.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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