Amelioration of Hemerocallis citrina total flavonoids on oxidative damage of liver cells HL-7702 / 中草药

ABSTRACT

Objective: To study the protective effects of Hemerocallis citrina total flavonoids (HCTF) on oxidative damage of liver cells HL-7702, and explore its possible mechanism. Methods: The liver cell damage model was induced by H2O2, and the proliferation of liver cells was examined by MTT assay, and the changes of ALT, LDH, and AST were dectected by ELISA assay, the levels of LXRα and FAS were determined by RT-PCR and Western blotting assay. Results: HCTF (5 and 10 μg/mL) could significantly improve the proliferation of liver cells (P < 0.01), the content of NO increased and the content of MDA decreased (P < 0.01 or 0.05), the activity of SOD increased (P < 0.05), the levels of mRNA, and protein of LXRα and FAS decreased (P <0.01 or 0.05). Conclusion: HCTF could inhibit the transcription and protein expression of LXRα and FAS through anti-oxidation, and repair the damage of HL-7702 liver cells caused by H2O2.