HPLC fingerprints of Polygonum hydropiper and its adulterants / 中草药

ABSTRACT

Objective: To study and establish the fingerprints of polygonilterba the aerial pevrts of Polygonum hydropiper by HPLC. Methods: The fingerprints of P·hydropiper from different origins were built using Hypersil ODS2 C18 column and acetonitrile-0.2%phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was set at 260 nm. The temperature of column was at 25℃. Total 22 Polygonum Herba samples and adulterants were detected. Results: The mutual mode to HPLC fingerprints was set up, and the 12 common peaks were obtained. The similarities in 15 batches of Polygonum Herba samples were all above 0.95 and those of seven batches of adulterants were below 0.50. Conclusion: The method is simple, stable, and reproducible, which can be uesd to identify Polygonum Herba and its adulterants.