Compatibility of adjuvant drug with others in Pingweisan Decoction on contents of honokiol and magnolol / 中草药

ABSTRACT

Objective: To develop an HPLC-DAD method for the detemination of the content change of honokiol and magnolol from classical Pingweisan (a prescription of Chinese materia medica used for stomach discomfort), and it is based on minister (Chen) medicine compatibility. Methods: After the decomposition of classical Pingweisan besed on Magnoliae Officinalis Cortex as minister medicine, 11 groups (one individual herb group, four compound groups, six groups with Magnoliae Officinalis Cortex as main medicine) were divided. The analysis was carried out on a GRACE AlltimaTM C18 (250 mm × 4.6 mm, 5 μm) column with a mobile phase which was composed of acetonitrile (B) and water (A) for isocratic elution. The column temperature was 25 ℃, the flow rate was 1 mL/min, and the detection wavelength was set at 294 nm. Results: The linear relationship of honokiol and magnolol is better within the ranges in 20.12-120.72 μg/mL (r = 0.999 0) and 20.16-120.96 μg/mL (r = 0.999 1); Study on the precision and repeatability, the RSD value of both honokiol and magnolol was less than 2%, which showed that the precision of the instrument and the method of repeatability were favourable. Stability test showed that the determination of the components was stable within 24 h. The recoveries were 98.69% and 101.28%, and the RSD values were 1.47% and 1.24%, which showed that the method had a good accuracy. Monarch Atractylodis Rhizoma, Zingberis, and Jujubae Fructus could make the contents of honokiol and magnolol in Pingweisan reduced; while the assistant Citri Reticulatae Pericarpium and guiding Glycyrrhizae Radix could make the contents increased. Conclusion: The other crude drugs in Pingweisan could affect and change the contents of honokiol and magnolol. It could explain the synergistic effect of compatibility and detoxification of the classical compound Pingweisan.