Cloning and sequence analysis of squalene epoxidase gene from Inonotus baumii / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2768-2773, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-853977
ABSTRACT
Objective:
To clone the full-length cDNA encoding squalene epoxidase (SE), which is the key enzyme involved in the triterpenoid biosynthesis pathway in Inonotus baumii, and analyze its bioinformates.Methods:
Taking total RNA as template, the full length cDNA and DNA of SE in I. baumii was cloned through RT-PCR and the rapid amplification of cDNA ends (RACE) technique. The bioinformatics of SE gene were analyzed by ExPASy on line.Results:
Sequence analysis showed that it consisted of 2145 bp with an open reading frame (ORF) of 1 452 bp, encoding 483 amino acid polypeptides. SE gene contained six exons and five introns. The relative molecular mass of SE calculated was 5.3 × 104, the isoelectric point (pI) was 8.41, and there was no signal peptide in SE.Conclusion:
It is the first report that the cDNA encoding SE from I. baumii is cloned. This work provides a scientific basis for exploring the triterpenoid biosynthesis pathway of the medicinal ingredient and improving its quality in I. baumii.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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