HPLC-UV fingerprint and medicinal quality evaluation of saponins from plants of varieties of Hedera helix / 中草药

ABSTRACT

Objective: Through establishing the HPLC-UV fingerprint of saponins from the plants of varieties of Hedera helix L., using cluster and principal component analysis methods to do statistical analysis and combining with the determination of the calibration compound to provide a reference for the medicinal quality evaluation on the plants of varieties of Hedera helix L. Methods: The chromatographic separation was performed on Unitary C18 column (250 mm × 4.6 mm, 3 μm); The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid solution; The elution mode was binary high pressure gradient system; The detection wavelength was 205 nm; The flow rate was 1 mL/min; The injection volume was 20 μL and the column temperature was 25 ℃. Results: Using the similarity evaluation system for chromatographic fingerprint of Chinese materia medica to calibrate 12 common peaks of 17 samples of varieties of Hedera helix L. and calculate their similarity between 0.715-0.992; The preliminary results is that the medicinal quality of ivy is better than the other varieties of varieties of Hedera helix L. by analysis of cluster and principal component. Conclusion: The HPLC-UV fingerprint can be used to screen the high quality raw materials of varieties of Hedera helix L. and do the quality evaluation and control, in the meantime, it can be used to provide a new reference of studying on the medicinal quality evaluation for the plants of varieties of Hedera helix L.