Determination of loganic acid, chiratin, and loganin in Dipsaci Radix / 中草药

ABSTRACT

Objective: To establish a method for the determination of loganic acid, chiratin, and loganin in Dipsaci Radix and improve the quality evaluation by analyzing Dipsaci Radix from different habitats. Methods: The analysis was performed on Venusil MP C18 column (250 mm×4.6 mm, 5 μm) with mobile phase consisting of 0.1% H3PO4-acetonitrile for gradient elution 0-10 min, 8%-9% B; 10-30 min, 9%-11% B; 30-35 min, 11%-100% B. The flow rate was 1 mL/min and the column temperature was 25℃. Results: The results showed that loganic acid, chiratin, and loganin were well separated with the good linearity in 18.4-368.2, 2.02-40.4, and 17.5-349.6 μg/mL, respectively. The average recoveries of the three iridoid glycosides were 99.34%, 99.19%, and 101.61%. Conclusion: Loganic acid, chiratin, and loganin are the main iridoid glycosides in Dipsaci Radix. The method can easily be applied to the content determination of loganic acid, chiratin, and loganin in Dipsaci Radix quickly. The content ranges of loganic acid, chiratin, and loganin are 20.4-186.8, 26.4-177.7, and 1.9-13.2 mg/g, respectively, and the sum of the three iridoid glycosides shows no significant differences in various Dipsaci Radix from different habitats, and proposes to evaluate the quality of Dipsaci Radix.