Chemical constituents in roots of Ilex kudingcha / 中草药

ABSTRACT

Objective: To investigate the chemical constituents in the roots of Ilex kudingcha. Methods: Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR, and 13C-NMR, and physical constants were used to elucidate their structures. Results: Nineteen compounds were isolated from 95% ethanol extracts of I. kudingcha, including α-aromadendrol-3β-palmitate (1), β-sitosterol (2), α-amyrin (3), 3-acetoxyolean-12-en-28-ol (4), 3-acetoxyurs-12-en-28-ol (5), 3β-acetoxyurs-12-en-28-al (6), oleanolic acid (7), ursolic acid (8), quercetin (9), daucosterol (10), ficuscarpanoside B (11), 3β-hydroxy-28-norurs-12, 17-dien (12), siaresinolic acid (13), pomolic acid (14), α-D-glucose (15), 2, 6-dimethoxy-benzoquinone (16), syringaldehyde (17), dibutyl phthalate (18), and ilexoside D (19). Conclusion: Compounds 6, 12, 16-18 are isolated from the plant for the first time. Compound 11 is isolated from Ilex L. for the first time.